In this case study, ARGEN was used to determine the effect of protein concentration on the aggregation propensity of bovine serum albumin under stirring stress.Read More
These rAAV capsid stability experiments investigated and monitored the solution behavior of full and empty capsids under thermal stress using ARGEN. ARGEN is uniquely suitable for in situ and real-time characterization of solution stability of larger species such as rAAVs and LNP-RNA complexes. This case study demonstrates ARGEN's ability to elucidate differences in full and...Read More
The successful development of biotherapeutics requires an in-depth understanding of the impacts associated with mechanical and physical stress. Instability and subsequent aggregation can render them biologically inactive or even induce an immunological response in patients. Stirring stress is one of the most notable factors which disrupts native biopolymer structure and promotes aggregation. However, contact stirring...Read More
On the limits of 90° static light scattering for determining weight average molar mass Mw A discussion with Chief Science Officer, Dr. Wayne Reed This Technical Note assesses the fractional error in Mw that results when θ=90° static light scattering detection is used, instead of a full angular extrapolation via MALS. Read More
In these experiments, ARGEN was used to characterize the thermal and chemical stability of a monoclonal antibody and subsequently establish quantitative and qualitative properties such as pH and temperature dependence on aggregation kinetics. Additionally, SEC elution profiles were superimposed onto Mw/Mo output, providing a robust comparison and identification of the stability landscape for the mAb....Read More
Understanding the thermal, chemical and mechanical stability and physical properties of polymers is essential for development success. The case studies below outline the experimental methodologies and data analysis using ARGEN™ to monitor polymer degradation and to characterize molecular weight as well as the effects of temperature, shear stress and solution conditions on the stability of...Read More
