This dynamic webinar will offer insights into characterizing the stability of an engineered adeno-associated virus (AAV) serotype under moderate thermal stress and physiological solution conditions using light scattering measurements. While AAVs are leading vector systems for gene therapy, significant gaps remain in the understanding of instability and subsequent ejection of DNA from AAV capsids resulting from various thermal, chemical and mechanical stress.

The webinar will highlight the experiments that investigated and characterized the effects of thermally-induced perturbations at three temperature setpoints on DNA ejection from a proprietary AAV vector and the determinations of absolute molecular weights of full and empty capsid proteins. Interestingly, it was found that the AAV capsid proteins aggregated immediately upon the ejection of DNA and full capsids with DNA aggregated very slowly. This is an indication of the potential role of DNA in maintaining capsid architecture and preventing capsid protein aggregation.

Defining quantitative and qualitative properties of viral vector systems is critical to ensure maximum efficacy and stability during processing, storage,  and parenteral administration. Click on the button below to register for this exciting webinar that will provide key implications toward optimizing the efficacy and storage conditions of adeno-associated virus pharmaceuticals. All registrants will also receive a recording of the live webinar after the event.

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Meet the Speaker: Prof. Wayne Reed