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ARGEN Technical Notes

ARGEN Digital Booklet

The ARGEN™ Digital Booklet provides insight into various applications utilizing ARGEN’s patented simultaneous multiple sample light scattering (SMSLS) technology to accelerate and optimize the development of novel biotherapeutics. Biopharmaceutical companies are experiencing greater demand to develop novel biologic therapeutics in less time, and this requires a paradigm shift in the way teams approach formulation development...
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Assessing Stirring Stress and Other Impacts of Bioprocessing

The successful development of biotherapeutics requires an in-depth understanding of the impacts associated with mechanical and physical stress. Instability and subsequent aggregation can render them biologically inactive or even induce an immunological response in patients. Stirring stress is one of the most notable factors which disrupts native biopolymer structure and promotes aggregation. However, contact stirring...
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Using 90° SLS to Determine Mw

On the limits of 90° static light scattering for determining weight average molar mass Mw A discussion with Chief Science Officer, Dr. Wayne Reed Technical Note 003 assesses the fractional error in Mw that results when θ=90° detection is used, instead of a full angular extrapolation via MALS.  
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Rapid Determination of Mw for a Monoclonal Antibody

Colloidal stability is determined by the balance of repulsive and attractive intermolecular interactions between protein molecules, like a monoclonal antibody, to conserve the native folded state. Simply stated, the propensity for aggregation is reduced by less intermolecular interaction. Therefore, determination of the second virial coefficient (B22) is a valuable screening tool to predict aggregation propensity....
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Kinetic Analysis of Protein Stability & Oligomeric State Transitions of Human Insulin

These experiments demonstrate the utility of ARGEN™ to define oligomeric states and transitions of Human Insulin under thermal stress. Time to dimerization and time to tetramerization measurements permitted the characterization of a stable dimer species, as well as insights into the mechanisms and kinetics of higher order oligomer formation. Whereas, SEC allows the characterization of...
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Characterizing the Thermal & Chemical Stability of a mAb

In these experiments, ARGEN was used to characterize the thermal and chemical stability of a monoclonal antibody and subsequently establish quantitative and qualitative properties such as pH and temperature dependence on aggregation kinetics. Additionally, SEC elution profiles were superimposed onto Mw/Mo output, providing a robust comparison and identification of the stability landscape for the mAb....
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Biopolymer Characterization & Polymer Degradation

Understanding the thermal, chemical and mechanical stability and physical properties of polymers is essential for development success. The case studies below outline the experimental methodologies and data analysis using ARGEN™ to monitor polymer degradation and to characterize molecular weight as well as the effects of temperature, shear stress and solution conditions on the stability of...
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Comparing Protein Stability

Biopharmaceutical companies isolate many types of proteins for use in experimental and therapeutic medicines. Unfortunately, when removed from their native environments, proteins become less stable. Unstable proteins are more prone to degradation from the primary stress mechanisms: mechanical and thermal (from manufacturing, storage and transport) and biological (from drug delivery). The challenge for biopharmaceutical companies...
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